Abstract

The growth of two wetland forage grasses Leptochloa fusca (L.) Kunth. and Echinochloa stagnina (L.) P. Beauv. was investigated under NaCl salinity regarding the morphological traits, plant chemical composition, photosynthesis and the antioxidant enzymes POD and CAT. Plants of both species were irrigated with 0, 50, 100, 200, 300 and 400 mM NaCl in a sand/perlite mixture of 1:1 (v/v). Salinity negatively affected morphological traits and plant biomass of both species, particularly E. stagnina. Soluble sugars and proline within limits, protein contents of the foliage were increased to different extents by increasing salinity, but insoluble sugar decreased. K⁺/Na⁺ ratio of shoot and root of both species was decreased as a consequence of Na⁺ accumulation and restricted K⁺ uptake. NaCl salinity adversely affected photosynthetic rate, transpiration rate, stomatal conductance and water use efficiency of both species particularly E. stagnina. Tolerance of L. fusca and E. stagnina to salinity stress can be based on stomatal closure and reduction in leaf area, in order to minimize water loss via transpiration and increase the activity of some antioxidant enzymes to detoxifying ROS generated during stress.

Salinization is increasing on a global scale, and tolerance to salinity during germination is critical for the establishment of plants and growing in saline soil. Seed was investigated. Laboratory experiment with completely randomized design comprising three replicates in Petri dishes was conducted. In each petri-dish fifty seeds were placed. to determine the salt effects on A laboratory experiment was carried out to assess the germination and early seedling growth response to salinity stress of, which are common medicinal species in Libya. Seeds of fenugreek (Trigonella foenum-graecum L.) were treated with various concentrations of NaCl (0, 50, 100, 150,200 and 300 mM L1). control was moistened with ten milliliters of distilled water. The salt stress decreased seed germination the response of Trigonella foenum-graecum to salt stress and water stress was evaluated at the germination stage. The severe reduction in germination percentage and particularly germination speed with prolonged lag period by moderate salinity level of 100 mM NaCl, suggests that Fenugreek is a salt-sensitive species during germination. Salinity reduced germination uniformity and germination synchrony and might delay start of germination but accelerates its termination with a consequent shortening of the time spread of germination. The recovery percentage was lower but speed of recovery was higher compared with the corresponding parameters of non-treated seeds. Recovery percentage was slightly improved with the increase in the level of salinity pretreatment.

Vulvovaginal candidiasis causes sufferers much discomfort. Phytotherapy with garlic has been reported to be a possible alternative form of treatment; however, it is unknown why patients report varying success with this strategy. Fresh garlic extract has been shown to down-regulate the putative virulence gene, SIR2 in C. albicans. Our study aimed to see if previous observations were reproducible for the gene responsible for Candidalysin (ECE1). Two clinical strains from patients with reported variable efficacy of using garlic for the treatment of vulvovaginal candidiasis were compared through biofilm assays and antimicrobial susceptibility. Real-time PCR was used to assess changes in gene expression when exposed to garlic. Treatment with fresh garlic extract and pure allicin (an active compound produced in cut garlic) resulted in a decrease in SIR2 expression in all strains. In contrast, ECE1 expression was up-regulated in a reference strain and an isolate from a patient unresponsive to garlic therapy, while in an isolate from a patient responsive to garlic therapy, down-regulation of ECE1 occurred. future studies that investigate the effectiveness of phytotherapies should take into account possible varying responses of individual strains and that gene expression may be amplified in the presence of serum.

 The aim of the present paper is to develop a generalized fractional kinetic equation involving generalized multi-variable Mittag-Leffler function. Using the Laplace transform, the solutions of the fractional kinetic equation are established in terms on general Mittag-Leffler function. The results obtained here are general in nature to yield a large number known and (presumably) new results as their special cases. 

The Aqaba complex located at the southeastern of Jordan, exposes coexisting mafic-felsic association typical of syn-to pre-plate collision magmatism. The bulk of the pluton is made of quartz monzonite, granite and granodiorite. New U-Pb zircon dating revealed a synchronous emplacement of the granite (605 ± 4.6 and 617±3.7 Ma), granodiorite (613 ±4.4, 612 ±3.6 and 611.8±4.9 Ma) and quartz monzonite (608± 5.4 Ma). The whole-rock geochemistry indicate that the source for the quartz monzonite, granite and granodiorites could have been a continental arc-derived from mantle sources and mature metagreywackes. Melting of this crustal material was subduction related , which could have been an enriched mantle-derived melt contaminated. The Aqaba complex is a part of a the Arabian-Nubian Shield (ANS) are juvenile in character, Neoproterozoic, including in addition five large plutonic suites ; Rahma , Darba , Rumman,Urf and Yutum suite. The genesis of these suites were likely induced by mantle-derived magma in the shallow crust while their spatially and temporally discrete emplacement at shallow levels was probably related to the (extensional) of lithospheric boundaries, which represent a feasible fertile source for such granitoids.

In this study, the effect of a dietary supplementation of Apium graveolens on growth performance, digestive enzyme activities and immune response of juvenile common carp (Cyprinus carpio) were evaluated. C. carpio, with an average weight at the beginning of the experiment of 6.5 ± 0.07 g, were supplemented with a celery (Apium graveolens) aqueous methanolic extract at a dose of 0% (control), 0.1%, 0.5% or 1% over 45 days. Every 15 days, respiratory burst, lysozyme and myeloperoxidase activity were measured, and at the end of the study, haematological responses, growth performance and digestive enzyme activity were investigated. Respiratory burst and myeloperoxidase activity was significantly improved in all treated groups compared with the control group (P ˂ 0.05). Lysozyme activity was highest in the 0.1% and 1% extract groups. At the end of the study, the final weight (FW), weight gain (WG) and specific growth rate (SGR) were significantly increased in the 0.1% group compared with the other groups. The feed concentration ratio was decreased in the 0.1% and 1% groups compared with the control (P < 0.05). Haematological parameters were affected by A. graveolens extract intake (P ˂ 0.05). The total white and red blood counts, haemoglobin content and haematocrit value were highest in the 0.1% extract group (P ˂ 0.05). However, the mean cell volume and mean cell haemoglobin concentration were decreased in the 0.5% group (P ˂ 0.05). Digestive enzyme activity (trypsin, amylase and lipase) in all experimental groups was significantly elevated compared with the control (P ˂ 0.05). These results indicated an immunostimulatory and growth-promoting effect and increasing digestive enzyme activity of C. carpio supplemented with an A. graveolens aqueous methanolic extract.

Abstract. The growth of lampenflora detracts the natural beauty of cave walls, and threatens their archaeological value.

This is a real problem in Malaysian caves, therefore, an attempt should be made to eliminate these unwanted microorganisms. Ultraviolet light destroys harmful microbes such as bacteria, yeast. molds, viruses and algae, and ultraviolet radiation is less toxic to cave dwellers such as birds, reptiles and visitors. So ultraviolet radiation can be a way to control microorganisms or reduce their numbers. In this study. we studied the effect of ultraviolet radiation on microorganisms isolated from selected Malaysian caves to control and eliminate them AlterS minutes exposure, 254mm UV-C effectively eliminated colonies of Pseudomonas aerginosa and Stenotrophomonas sp. But P. guilliermondi and

R. dairenensis were eliminated to the zero after 30 mivutes of treatment, but C. Liquefaciens needed 60 minutes to be treated. Synechochococcus sp and Micractinium sp. fell to zero after exposure to 240 of UV-C

This study was conducted to determine the effect of dietary supplementation with Tilia tomentosa on the growth performance, digestive enzyme activity, haematological indices and nonspecific immune indices of juvenile common carp (Cyprinus carpio). Fish with an average weight of 4.35 ± 0.16 g were fed a diet supplemented with an aqueous methanolic extract of T. tomentosa at a dose of 0% (control), 0.01%, 0.05% or 0.1% for 45 days. The final weight, weight gain and specific growth rate were observed to be significantly higher for the 00.1% and 0.1% groups compared with the control group (P < 0.05). The feed conversion ratio was significantly decreased in the 0.05% and 0.1% groups compared with the control (P < 0.05). The activities of various digestive enzymes (amylase, lipase and trypsin) were also measured and no significant differences were observed compared to the control (P > 0.05). The mean cell volume of the 0.01% group was significantly increased compared to the control (P < 0.05) and increased lysozyme activity was observed in the 0.05% and 0.1% groups. Respiratory burst activity was significantly increased (P < 0.05) on days 15 and 30 for the 0.1% and 0.05% groups, respectively. No differences were observed for myeloperoxidase activity among the four groups. These results suggest that aqueous methanolic extract of T. tomentosa has a growth-promoting and immunostimulatory effect on common carp.

ABSTRACT

The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).