Coxiella burnetii (C. burnetii) is one of the most important bacterial agents that cause abortion (Q fever) in ruminants and is a zoonotic disease. The current study reported the first confirmed detection of C. burnetii in sheep in Libya using real-time polymerase chain reaction coupled with high-resolution melt analysis.

Case Description:

Two flocks of sheep suffered abortion, with the aborted fetuses varying in age from early to late term. Vaginal swabs were collected, and DNA was extracted. DNA samples were analyzed using real-time PCR coupled with high-resolution melting (HRM) analysis, and the results showed infection with the zoonotic Q fever caused by C. burnetii. The animals responded to treatment with the antibiotic enrofloxacin, and the abortion was stopped after treatment.

This study aimed to evaluate the effect of Artemisia herba alba extracts (aqueous, alcoholic and essential oil) on

antibiotic-resistant bacteria. In which four types were tested, namely Staphylococcus aureus (MRSA)

Staphylococcus epidermidis, Klebsiella pneumoniae, Klebsiella oxytoca, using the diffusion disk method and

concentrations (100, 200, 300 mg/ml) for the aqueous and alcoholic extract, while the essential oil was used in

its pure form. The most effective antibacterial activity was observed for the essential oil of plant with an inhibition

diameter from 8.00±0.00 to 11.33±1.52 mm, and its aqueous extract effect on bacteria MRSA and S. epidermidis

was 8.66±1.52 and 8.33±0.57 mm, respectively, at 300 mg/ml. The inhibitory effect of alcoholic extract was

10.00±0.00 mm at 300 mg/ml for MRSA as for S. epidermidis at the same concentration had high inhibition

diameter 13.66±1.15 mm. Phytochemical screention of aqueous extract revealed the presence of phenols and

saponins, whereas, alcoholic extract revealed the presence of phenols, tannins, terpenoids and saponins. The

chemical components of the essential oils were determined by GC-MS device. The main compounds of A. herba

alba were Camphor (30.527%), Thujone (22.471%) and Camphene (10.291%). These results suggest that

essential oil extract can be considered as a potential source of antibacterial compounds.

A highly efficient and accurate numerical method for systems of fractional differential equations (FDEs) with rational order is presented in this paper. Rational power functions and rational Taylor series projection are utilized to obtain approximate solutions. Rational semi-smooth spaces are introduced, and the regularity of solutions in these spaces is established. A series of theoretical results, such as the existence and uniqueness of solutions, properties of the rational Taylor series and its remainder term, and an operational matrix approach, are derived. It is proven that the numerical solution is exact when the exact solution is a rational power series, and the approximate solution is shown to be the rational Taylor series projection of the exact solution. The convergence of the method is analyzed. The efficiency of the proposed method is demonstrated through numerical experiments, which show significant improvements in computational time compared to existing methods.

In the past few years, the area of mathematical study has made considerable advances, owing largely to the introduction of artificial intelligence (AI) tools. Among these, artificial neural networks (ANNs) have played an important role in modernizing several mathematical techniques and problem-solving approaches. ANNs have recently become popular as a powerful mathematical research tool, providing an effective alternative to established approaches for solving fractal-fractional differential equations (FFDEs). This paper describes the use of a feed-forward ANN with a hidden layer to address systems resulting from the fractal-fractional Bagley-Torvik differential equation (FFBTDE). In addition, a power series (PS) technique is introduced to increase efficiency. The paper looks at for solving FFBTDE with variable and constant coefficients. The numerical findings show that the suggested strategy not only produces results that closely match exact and reference solutions, but also outperforms existing methods in terms of accuracy.

Artemisia herba-alba Asso is a medicinal plant renowned for its therapeutic essential oil. This study characterized the chemical profile and bioactivities of the essential oil from Libyan A. herba-alba. The essential oil, obtained via hydrodistillation with a 2% yield, was analyzed by GC-MS/FID and evaluated for antibacterial (disc diffusion) and anticancer (MTT assay) properties. The oil was dominated by camphor (30.53%) and Thujone (22.47%), classifying it as a camphor-thujone chemotype. It exhibited remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA; 17.33±1.52 mm), and demonstrated potent, dose-dependent cytotoxicity against MCF-7 breast cancer cells with an IC₅₀ of 0.59 mg/mL. The results validate the traditional use of this plant and highlight its potential as a source of antimicrobial and anticancer agents

Artemisia herba-alba Asso is a medicinal plant renowned for its therapeutic essential oil. This study characterized the chemical profile and bioactivities of the essential oil from Libyan A. herba-alba. The essential oil, obtained via hydrodistillation with a 2% yield, was analyzed by GC-MS/FID and evaluated for antibacterial (disc diffusion) and anticancer (MTT assay) properties. The oil was dominated by camphor (30.53%) and Thujone (22.47%), classifying it as a camphor-thujone chemotype. It exhibited remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA; 17.33 ± 1.52 mm), and demonstrated potent, dose-dependent cytotoxicity against MCF-7 breast cancer cells with an IC₅₀ of 0.59 mg/mL. The results validate the traditional use of this plant and highlight its potential as a source of antimicrobial and anticancer agents.

Abstract

This study investigated the allelopathic potential of aqueous extracts from two Libyan Artemisia species, Artemisia judaica and Artemisia campestris, on the seed germination and germination rate of Lactuca sativa L. Aerial parts of the plants were collected during the flowering stage in February 2020 from Zintan, Libya. Qualitative phytochemical analysis of the aqueous extracts revealed the presence of tannins, flavonoids, and saponins in both species, while phlobatannins and terpenoids were absent. A bioassay demonstrated that both A. judaica and A. campestris extracts exhibited significant allelopathic activity, inhibiting Lactuca sativa seed germination and decrease the germination rate (GR) in a dose-dependent manner. Complete inhibition of germination (100% IP) was observed at concentrations of 10 mg/ml and 20 mg/ml for both species. The findings suggest that the observed allelopathic effects are likely due to the identified phytochemical constituents, and that these Artemisia species could serve as promising sources for natural herbicides in agricultural weed control.

Background In arid regions such as Jennawen, Libya, groundwater is the primary source for drinking and irrigation, yet it is highly vulnerable to geogenic and anthropogenic contamination. This study aimed to assess the chemical and microbiological quality of 26 groundwater samples from rural and remote, including desalinated water. Material and Methods Physico-chemical parameters (e.g. pH, salinity, major ions) and microbiological indicators (total coliforms, E. coli, total bacterial counts) were analyzed according to established standards. Results revealed that most untreated groundwater samples exceeded the permissible limits for salinity, hardness, and sulfate. Furthermore, microbial bacterial contamination was found at various locations. While some RO desalinated water was free from microbiological contaminants, it was severely deficient in essential minerals. Conclusion The groundwater in the investigated area requires proper attention. Research and management strategies are critically needed to address both chemical and microbial contamination while maintaining an optimal mineral balance in potable water.

The genus Thymus is well-known for its aromatic and medicinal properties. Among its species, Thymus algeriensis is widely distributed in the Mediterranean basin, including Libya, where environmental conditions can influence essential oil composition and bioactivity. This study aimed to characterize the chemical profile of T. algeriensis essential oil collected from Gaser Alhaj and evaluate its antibacterial and allelopathic activities. Essential oil was extracted by hydrodistillation and analyzed using GC-MS, revealing six major compounds, with carvacrol as the dominant constituent (77.01%), followed by γ-terpinene, thymol, o-cymene, p-cymene, and caryophyllene. Oxygenated monoterpenes constituted the majority of the oil. The oil exhibited strong antibacterial activity against Escherichia coli and Salmonella gallinarum, and demonstrated dose-dependent allelopathic effects on Lactuca sativa seed germination. Compared to previous studies from Libya, this Gaser Alhaj oil represents a distinct carvacrol-rich chemotype with superior biological activity, highlighting regional chemotypic variation. These findings suggest that T. algeriensis essential oil has promising potential as a natural antimicrobial agent and eco-friendly bioherbicide