Abstract: The current study was aimed for isolation, identification and preservation of mycobiota associated with the olive oil processing wastes (Fatura) collected from different cities in Al-Gabal Al-Gharby, Libya, screening the cold-active lipolytic activity of the isolated fungi and selection of the highest cold-active lipase producers. 31 fungal species belong to 12 genera were isolated from these samples with total CFUs of 29560. Fusarium was the most common genus at total CFUs of 9020 and comprising 30.51% from all fungi, followed by Aspergillus, that recorded 25.44% from all fungi. Penicillium was ranked third, nine different species were present. A total of 100% of samples were found to have CFUs of 5140 and 17.4% of all fungi. On lipase production agar medium at two temperatures, 10 and 20°C, 102 fungal isolates from 31 species were tested for their lipolytic activity. The majority of fungi could produce lipase activity at 20°C, where 98 out of 102 isolates the highest lipase producers was higher at 10°C (25) than at 20°C (16). The most active isolates were Alternaria, Fusarium, and Penicillium. Molecular identification of the most active four isolates was carried out by sequencing their internal transcribed spacer region (ITS).

 Keyword: Olive oil, cold active enzymes, Lipase, fungi, lipolytic activity, Fatura.

Abstract: This study aims to analyzing demographic data of patients, isolate and identify Candida species, which causes vaginal infections, and a study of its prevalence among pregnant women in Sorman city, Libya.210 specimens collected from patients admitted to the Maternity Care Center in the combined clinic. carried immediately to the Microbiology Laboratory in the National Cancer Institute, Subrata, Libya for direct microscopy, culturing, and characterization. Each participant was given an interview questionnaire and asked about their age, educational level, employment position, and history of recurrent vaginal yeast infection. Chronic diseases were also listed on the data collecting form. Identification of Candida species using Chrome agar: A total of 100 isolates have been recovered in this study, of which 72isolates were obtained as pure cultures on Chrome agar medium. According to their color on Chrome agar, these 72 colonies were categorized to 5 main species namely Candida albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis. Genotypic identification of Candida species in this investigation was validated by the ITS tree. Eight strains from this investigation were found in the Candida albicans clade, which had a high bootstrap value of 99 percent ML/99 percent MP. These were therefore identified as Candida albicans., Within the Candida glabrata clade, three isolates were grouped together, demonstrating a strong support value of 99% ML/99%MP. These strains were recognized as belonging to the C. glabrata species, while one isolate was recognized as belonging to the C. tropicalis species, with a high support value of 99% ML/99%MP.

 Keywords: VVC infections, candidemia patients, genotypic identification, and Candida albicans

Abstract—The majority of Neoproterozoic rocks exposed in southern Libya, are comprised of intrusive coarse-grained porphyritic, two-mica, and high K-calc alkaline granite. The Jabal Fezzan Granite (JFG) is located in southwestern Libya. In this study, trace elements and whole-rock geochemistry have been used to understand the origin and the process of petrogenesis of the studied granites. The JFG is high-Si, Rb, Y, Nb, and (ASI values greater than 1.1). Mineralogically, it is characterized by the presence of minor muscovite and biotite and a lack of hornblende, exhibiting features of S-type granites, and having a character that belongs to an alkali-calcic series. According to geochemical value, light REE-enriched, characterized by moderate

enrichments in LREE (La/Sm), HREE, and weak negative Eu-anomalies. The geochemical modeling of the reveals that the JFG derived from the melting of the crust and underwent high fractional crystallization of plagioclase and K-feldspar at (H-P) conditions (750–980°C/1–4 GPa). The Jabal Fezzan (JFG(~50%) ) formed during Pan-African orogenic events during the destabilization of the interior Saharan metacraton due to compression stress and transpressive movements along pre-existing weakness and reactivation of shear zones inherited from Paleoproterozoic evolution. The Neoproterozoic basement forms the northernmost margin of the intracratonic Muruzq Basin, as evidenced by (greenschist facies) and intruded granitic rocksderived at the syn-collision stage (630–540 Ma)

(JFG) .

The primary aim of the current study was to

evaluate and compare antioxidant activity in

leaves and flowers extracts of arbutus pavarii.

For the purpose, the antioxidant potential of

crude methanol extracts of arbutus pavarii was

screened in vitro using reducing power,

phosphor molybdenum assay, and radical

scavenging activity by employing DPPH, NO,

.OH methods as well as ferric thiocyanate (FTC)

and thiobarbituric acid (TBA) tests to confirm

the antioxidant potential of these extracts.

Resultantly, it was confirmed that the leaves

extract (LE) has displayed higher reducing

ability compared with flowers extract (FE). The

maximum antioxidant activity was found it in

leaves methanol extract (199.38±12.73 mg of

ascorbic acid/g of dry weight). The Methanolic

LE was able to reduce the DPPH concentration

with an IC50 of 1.09 ± mg/mL, which was

noticeable stronger (P < 0.01) than that of the

positive control (ascorbic acid), (IC50 = 0.01 ±

1.6 mg/mL) and FE (IC50= 1.25 ± mg/mL) as

well. The LE showed slightly inhibited .OH

radical (IC50, 0.78 mg/mL) compared with FE

(IC50, 0.91 mg/ml). The obtained results of this

investigation indicated the usefulness of

utilization of arbutus pavarii leaves as a reliable

source of antioxidants for nutritive and

industrial purposes.

ABSTRACT - This study aims to determine and investigate the provenance of the source rocks and the depositional setting of the Kikhla Formation, Lower Cretaceous, northwestern Libya. It is mainly composed of mature quartzitic sandstone alternating with conglomerate and clay. The study proved that the sandstone is yellow-white in color and commonly carries quartz granular and small fragments of silicified wood. The environmental deposition is under differing fluvial conditions and by a large braided river. Texturally, the Kikhla sandstone is poorly cemented, coarse to very coarse grained and strongly unimodal and is classified as litharenite and subarkose by the modal composition, which is supported by geochemical studies. According to petrography and geochemistry, the Kikhla sandstone was deposited in a passive continental margin basin, mainly from granitic sources. On the basis of major elements

ratios, and petrographic interpretation, the source rocks are most likely recognized as granites that were exposed via rifting. The CIA

(Chemical Index of Alteration) values (73.28-93.97) indicate a high degree of chemical weathering, which could be attributed to the

arid climate conditions in the source area and mainly controlled by the source-rock provenance, hydraulic sorting during transport and

deposition, diagenesis and depositional environment. According to chemical investigations, sandstone exhibits significant

concentrations of SiO2, Na2O>K2O, and Fe2O3, which is compatible with the modal data.

Keywords: Active continental margin. Kikhla Formation. Quartzitic sandstone. Chemical weathering. Hydraulic sorting. Source-rock provenance.

In this present work, the effect of variation of parts (pulp and peel) on photochemical 

compounds such as phenolics and flavonoids content of the banana flours were 

studied. The results exhibited that different varieties did exhibit significant differences 

on TPC as well as TFC which means that different sample yielded different values of 

TPC and TFC. The TPC was determined in ethanolic extracts of banana peel and pulp 

flour. The TPC was found to be about 0.40 mg/ml in banana peel and about 0.33

mg/ml in banana pulp. Thus, it is clear that the TPC was higher in the peel than in the 

pulp, while the total flavonoids content was determined in methanolic extracts of 

banana peel and pulp flour. The results showed that TFC was higher in banana peel 

(0.37 mg/ml) than in banana pulp (0.20 mg/ml). 

Key words: banana, catechol , quercetin, total flavonoids, total phenolics.

AbstractIntroduction

The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives

This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and Methods

The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results

The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion

This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.

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